Aim To investigate the effect of resveratrol (Res)on septic mice and LPS-insulted H9c2 cells,as well as its involved mechanisms.Methods By use of a mouse cecal ligation and puncture-induced septic model (CLP),the survival of septic mice was evalua-ted after resveratrol treatments.H9c2 cells were insul-ted by LPS and then treated with resveratrol,the mR-NA expressions of TNF-α,SIRT1 and other class III HDAC members were detected using RT-PCR and real-time PCR,Finally,the protein levels of nuclear p65, an important subunit of NF-κB,were measured in H9c2 cells using Western blot assay,to reveal the effect of resveratrol on LPS-induced nuclear transloca-tion of NF-κB.Results Compared with the control septic animals,intraperitoneal injection of resveratrol (1 or 5 mg·kg -1 ·d -1 )significantly increased the survival of septic mice.Furthermore,resveratrol signif-icantly increased mRNA expressions of SIRT1,SIRT2, SIRT6 and SIRT7 in LPS-insulted H9c2 cells.Res-veratrol also remarkably inhibited LPS-induced nuclear translocation of NF-κB.Conclusion An appropriate dose of resveratrol protects septic mouse hearts from the injury induced by LPS through the activation of SIRT family members and the inhibition of NF-κB pathway.%目的：从动物休克模型和细胞水平上研究白藜芦醇（resveratrol，Res）对败血症的调节作用及其分子药理学作用机制。方法通过建立小鼠盲肠结扎穿孔诱导的败血症休克模型（CLP），研究白藜芦醇给药后的小鼠的存活率。然后利用体外培养的心肌细胞建立 LPS 损伤模型，采用反转录PCR（RT-PCR）、Real-time PCR 检测细胞内源性 TNF-α、SIRT1等的 mRNA 的表达水平，Western blot 分析 NF-κB 重要亚基的蛋白表达水平。结果与单纯休克模型组相比较，腹腔注射白藜芦醇（1和5 mg·kg －1·d －1）治疗明显增加败血症休克小鼠的存活率；白藜芦醇处理明显促进 SIRT1、SIRT6和 SIRT7的 mRNA 表达，抑制 LPS 诱导的 NF-κB 核转位。结论适当剂量的白藜芦醇可能通过上调 SIRT 家族成员，并抑制 NF-κB 炎症反应通路，对败血症休克具有潜在的药理学保护作用。
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