目的 研究旨在观察针刺对海洛因成瘾戒断大鼠焦虑相关行为是否有作用,并分析其可能机制.方法 按逐日递增的原则皮下注射海洛因建立成瘾模型,停止给药后纳洛酮催瘾,建立海洛因成瘾戒断动物模型;连续7 d针刺"神门"穴;通过高架十字迷宫实验观察戒断大鼠焦虑程度及针刺的抗焦虑作用;以放射免疫分析技术(RIA)测定大鼠血浆中皮质醇(corticosterone,CORT) 、促肾上腺皮质激素 (adrenocorticotropin hormone,ACTH) 和精氨酸加压素(arginine vasopressin,AVP)水平.应用免疫组织化学检测下丘脑室旁核(paraventricular nucleus,PVN)反应神经元活动度Fos蛋白表达.结果 针刺明显增加海洛因成瘾戒断大鼠进入开臂的次数百分率及在开臂滞留时间百分率(P<0.01或P<0.05);放免结果显示针刺降低海洛因成瘾戒断大鼠血浆CORT、ACTH和AVP水平(P<0.01或P<0.05);免疫组化结果显示针刺降低戒断大鼠下丘脑室旁核Fos蛋白表达(P<0.01或P<0.05).结论 针刺降低海洛因成瘾戒断大鼠焦虑程度和调节下丘脑-垂体-肾上腺(hypothalamic-pituitary-adrenal,HPA)系统活动,提示针刺可以缓解海洛因成瘾戒断期间所致的情绪异常,其机制可能与调节下丘脑-垂体-肾上腺系统功能有关.%Aim To assess the effect of acupuncture on anxiety-related behaviors after heroin-withdrawal, and explore its possible mechanism. Methods Heroin was injected subcutaneously according to the principle of daily increasing dose in rats of experimented group. The abstinence signs were precipitated by naloxone after drugs were stopped, and the animal model of heroin addiction was established. Acupuncture was continuously given for 7 days. The elevated plus maze test was used to observe anxiety level after heroin-withdrawal and the anti-anxiety effect of acupuncture. The concentration of corticosterone ( CORT ), adrenocortico-tropic hormone ( ACTH ) and arginine vasopressin ( AVP ) in the plasma of the rats were detected by radio-immunity. Expressions of Fos protein in the para-ventricular nucleus of rat hypothalamus were detected by immunohistochemistry method. Results Acupuncture significantly increased the percentage of entries in-to open arms and time spent on open arms( P <0. 01 or P < 0. 05 ). The radioimmunoassay results showed that acupuncture decreased plasma CORT, ACTH and AVP in heroin-withdrawal rats( P < 0. 01 or P < 0. 05 ). The immunohistochemistry results showed that the expression of Fos protein in hypothalamic paraventricular nucleus was decreased after acupuncture ( P < 0. 01 or P < 0. 05 ). Conclusions Acupuncture reduces anxiety level of heroin-withdrawal rats and modulates the hypothalamic-pituitary-adrenal system activities. These findings suggest that acupuncture at "shenmen" point may be useful as a therapeutic treatment in heroin withdrawal induced emotional disorders, and its mechanism may be related to modulating the activities of hypothalamic-pituitary-adrenal system.
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