为探索不同栽培方式对白肋烟烟碱转化率及烟草特有亚硝胺(TSNA)含量的影响,采取裂区试验,以不同烟碱转化率类型的品系为主处理,不同种植规格、行向为副处理和再副处理,进行了垂垄向和垄向烟叶的光照强度、生物碱含量、烟碱转化率及4种TSNA含量的比较分析.结果表明,不同转化率的品系是影响烟碱转化率和TSNA含量的主要因素;不同种植规格、行向及不同方向烟叶的TSNA含量存在显著的差异;品系与行向及种植规格间亦存在显著的互作效应,且HC(高转化)品系受行向和种植规格的影响大于LC(低转化)品系.初步推断,生物碱代谢、TNSA的形成与积累均与光照强度有关.据此认为,在白肋烟生产上首先要选用低转化率品系,确立合理的栽培规格及行向,努力改善烟株的光照条件,是降低烟叶TSNA含量的有效措施.%In orderto explore the effects of different cultivation methods on burley tobacco nicotine conversion rate and TSNA content,A split plot experiment with three replicates was designed to determine light intensity,alkaloid content,nicotine conversion and four kinds of TSNA content in a comparative analysis.The results showed that,conversion rate difference between varieties is a major factor affecting TSNA content.Significant influence of TSNA content was also observed by planting with different specifications,and perpendicular to the ridge or parallel on the ridge.There is also a significant interaction effect between variety and row direction and planting specification.HC varieties were affected more significantly by planting specifications than LC varieties.Formation and accumulation of alkaloid metabolites and TNSA are likely affected by light intensity.Effective measures to reduce the content of TSNA in tobacco leaves include using low conversion rate varieties,good cultivation practices and appropriate row direction to improve light conditions of tobacco plants.
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