目的：探讨二氧化硒（SeO2）对宫颈癌细胞株Hela凋亡的诱导作用及其对凋亡相关蛋白半胱氨酸天冬氨酸蛋白酶3（caspase-3）、P53表达的影响。方法用光学显微镜观察Hela细胞经过SeO2处理24h后的形态学变化；用四甲基偶氮唑蓝（MTT）比色法检测SeO2对Hela细胞增殖及活力的影响；利用流式细胞术（FCM）检测Hela细胞凋亡率；用Westernblot检测Hela细胞内caspase-3、P53蛋白表达的变化。结果光学显微镜下SeO2可对Hela细胞生长形态产生明显影响，大量细胞变圆皱缩，失去正常形态，贴壁细胞减少，增殖减慢；MTT结果显示SeO2对Hela细胞的抑制作用呈量效依赖关系，其中7．500、15．000、30．000μmol/L组与对照组比较，差异有统计学意义（P＜0．05）；0、1．875、3．750、7．500、15．000、30．000μmol/LSeO2诱导Hela细胞凋亡率分别为3．12％、30．56％、33．42％、37．50％、45．43％、69．38％，呈明显上升趋势。Westernblot分析显示SeO2可上调caspase-3与P53蛋白水平，并于7．500μmol/L处达到峰值。结论SeO2可能通过上调caspase-3、P53的表达诱导宫颈癌细胞凋亡。%Objective To investigate the inducing effects of selenium dioxide(SeO2 ) on the apoptosis in human cervical carcino-ma cell line Hela and its influence on the expression of apoptosis-related proteins caspase-3 and P53 .Methods Hela cells were trea-ted with different concentrations of SeO2 for 24 h in vitro ;the morphological changes of Hela cells were observed by the optical mi-croscope;the influence of SeO2 on the cell proliferation and vitality was examined by the MTT assay ;the flow cytometry was em-ployed to detect the cell apoptosis rate ;the expressions of caspase-3 and P53 proteins in Hela cells were determined by the Western blot analysis .Results Under the optical microscopy ,SeO2 generated the obvious influence on the cell growth morphology ,a large number of cells became rounded and shrunken ,and lost the normal form ,while the adherence cell number was evidently decreased and the proliferation was slowed down ;the MTT results showed that SeO2 markedly inhibited the cell proliferation and viability in a dose-dependent manner ,in which ,the cell apoptosis rates induced by the 0 ,1 .875 ,3 .750 ,7 .500 ,15 .000 and 30 .000 μmol/L con-centrations of SeO2 were 3 .12% ,30 .56% ,33 .42% ,37 .50% ,45 .43% and 69 .38% respectively ,which revealing the obviously in-creasing trend;the Western blot assay revealed that SeO2 could up-regulate the caspase-3 and P53 levels ,and reached the peak value at the concentration of 7 .500μmol/L .Conclusion SeO2 could induce the cervical cancer cell apoptosis possibly by up-regulating the expressions of caspase-3 and p53 in Hela cells .
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