目的 检测特应性皮炎(AD)患者外周血单个核细胞(PBMC)中miRNA-142-5p的表达情况,并对其进行潜在靶基因预测.方法 选择2011年10月至2012年2月该院门诊皮肤科AD患者8例为观察组,与观察组年龄和性别相匹配的健康体检者6例为对照组.提取分离所有受试者PBMC中的miRNA,采用实时荧光定量PCR法,检测miRNA-142-5p的表达水平,运用miRanda、PicTar和TergetScan 等靶基因预测分析软件等生物信息学方法,筛选其潜在靶基因.结果与对照组比较,miRNA-142-5p 在观察组患者PBMC中的表达水平上调(P＜0.05),预测到交集靶基因有 39个,涉及到信号转导、细胞凋亡、细胞分化等.结论 miRNA-142-5p在 AD患者PBMC中高表达,可能通过调控其下游靶基因在AD发病中起着重要作用.%Objective To detect the expression levels of miRNA-142-5p in peripheral blood mononuclear cell (PBMC) from the patients with atopic dermatitis (AD) and to predict its latent target genes .Methods 8 cases of AD treated in the dermatological outpatient department of this hospital from October 2011 to February 2012 were selected as the observation group and 6 age-matched and sex-matched individuals with physical examination as the control group .PBMC were isolated .miRNAs in PBMC were extracted and separated by miRNA extraction and separation kit .The expression levels of miRNA-142-5p in PBMC were detected by using real-time quantitative PCR .Six databases including miRanda ,PicTar and Targetscan were adopted for predicting the targets of selected miRNAs .Results Compared with the control group ,the relative expression levels of miRNA-142-5p in PBMC of AD patients were up-regulated (P<0 .05) .A total of 39 target genes for miRNA-142-5p were shared between these five databases , which might be involved in signal transduction ,apoptotic process and cell differentiation .Conclusion miRNA-142-5p is up-regulated in the PBMC of AD patients ,which may play an important role in the pathogenesis of AD by regulating the downstream target gene.
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