目的 通过观察芪榆油膏对肛瘘术后创面肉芽组织中表皮生长因子(EGF)mRNA表达的影响,探讨其分子生物学水平的作用机制.方法 选择2009年6月至2010年3月贵阳中医学院第一附属医院肛肠科病房低位单纯性肛瘘患者90例,随机分为观察组、对照组和凡士林组,每组30例.采用RT-PCR方法 对术后用药第7天各组创面肉芽组织中EGF mRNA的表达进行检测并比较各组疗效.结果 观察组术后用药第7天创面肉芽组织中EGF mRNA基因表达均明显超过对照组和凡士林组(P ＜0.01);创面愈合时间观察组明显少于对照组和凡士林组(P＜0.05).结论 芪榆油膏对瘘术后肛创面的愈合作用可能通过上调EGF mRNA的表达介导.%Objective To observe the influence of Qi Yu ointment on the expression of epidemal growth factor( EGF) mRNA *in anal fistula wound granulation tissue to explore its mechanism of molecular biology level.Methods 90 patients with simple low anal fistula were randomly divided into three groups : observation group, control group and vaseline groups with 30 patients respectively.And then detect the expression of vEGF mRNA in each group which has received treatment for 7 days by RT-PCR method and then compare the efficacy of each group.Results The expression of FGF mRNA in the observation group was higher than that of control group and vaseline group(P＜0.01) ;and wound healing time of the observation group was shorter than that of the control group and the vaseline group(P＜0.05).Conclusion The function of Qi Yu ointment on the Qi Yu ointment of the wound tissue is conducted by EGF.
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