目的:建立复方薰衣草油膏中薄荷脑含量测定的方法.方法:采用气相色谱法,色谱柱为以改性聚乙二醇为固定相的毛细管柱(30 m×0.32 mm,0.25 μm);柱温为程序升温至230 ℃,分流比5∶1;进样口与检测器温度均为250 ℃.结果:薄荷脑质量浓度在0.022 67～0.680 10 mg/ml范围内呈良好的线性关系(r=0.999 5),平均回收率为98.36％,仪器精密度、中间精密度RSD分别为0.64％、1.43％.结论:该方法简单、可行、准确、重现性好,适用于复方薰衣草油膏中薄荷脑的质量控制.%Objective:To establish a method for content determination of menthol in compound lavender oil ointment by gas chromatography(GC).METHODS: GC method was adopted,capillary column(30 m×0.32 mm,0.25 μm)was used with modified polyethylene glycol as stationary phase.The column temperature was at 230 ℃ with the split ratio of 5∶1.The temperature was set at 250 ℃ for injection port and for the detector.RESULTS: The liner range of menthol was 0.022 67 mg/ml-0.680 10 mg/ml(r=0.999 5)with the average recovery of 98.36％,and the RSD value for instrument precision and intermediate precision were 0.64％,1.43％respectively.CONCLUSIONS: This method is simple,feasible,accurate,reproducible,which can be used for quality control of menthol in compound lavender oil ointment.
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