To fully understand the microbial diversity of goat milk,the microbial composition of the goat milk from Yangling,Baoji and Weinan region of Shaanxi province were studied using the 16S rRNA sequencing method targeting at the V3-V4 region.A total of 225 424 high-quality sequences were obtained,with average of 673 Operational taxonomic units (OTUs) per sample.The analysis of microbial composition showed distribution in 27 phyla.Proteobacteria dominated the microbiota in all goat milk samples.It accounted for 91.15％ of total population in the sample from Weinan region.Also,Proteobacteria accounted for 62.44％ and 60.33％ in samples from Yangling and Baoji area respectively.There were 354 genera,and the dominant genus,Enterobacter,which account for 37.41％,33.82％ and 2.87％ in samples from Weinan,Yanglin and Baoji area respectively.At species level,Pseudomonas aeruginosa dominated within the 826 species.It accounted for 7.96％,4.34％ and 3.20％ in samples from Weinan,Yanglin and Baoji area respectively.The composition analysis showed the highest microbial diversity in goat milk from Yangling and Baoji.The bacterial composition distribution in goat milk differentiated at certain degree.However,the overall differences were small,and they all exhibited substantial similarities.%为全面了解羊乳中细菌多样性,对陕西关中地区杨凌、宝鸡和渭南3个牧场羊乳样品中微生物宏基因组DNA的16S rRNA基因V3-V4区进行扩增、高通量测序.研究共获得225 424条高质量序列,平均每个样品聚成673个OTUs.对不同地区羊乳中细菌群落构成分析显示,所有羊乳样品中细菌门有27种,Proteobacteria为陕西关中地区羊乳中的优势菌群门,在渭南地区所占比例最高,为91.15％,在杨凌和宝鸡地区分别占62.44％和60.33％;细菌属有354种,Enterobacter是关中地区羊乳中的优势细菌属,3个地区所占比例分别为37.41％、33.82％和2.87％;细菌种有826种,P.aeruginosa为陕西关中地区的优势菌株,在3个地区分别占1.17％、0.81％和21.8％,且渭南地区的P.aeruginosa含量显著高于其他两个地区.不同地区羊乳样品细菌群体间的多样性分析表明,杨凌和宝鸡这两个地区羊乳中细菌群落丰富度和多样性较高,渭南地区羊乳中细菌群落丰富度和多样性较低.3个地区间羊乳中细菌分布有一定的差别,但样品之间微生物群落结构差距不大,有一定的相似性.
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