用微波辅助乙醇回流法从金针菇中提取抗氧化物质,利用正交试验对提取工艺参数进行优化研究.利用DPPH法检测提取物的清除自由基能力,选择对清除率有显著影响的3个因素:微波温度、微波时间、料液比,做了3因素3水平的正交分析实验,得出适宜工艺为:微波温度75℃,微波时间23 min,料液比1∶6.在此条件下自由基清除率达到了95.60%.通过研究金针菇提取物的抗氧化性能,证明金针菇提取物具有较强的清除自由基DPPH·的活性和还原能力,而且对处在不同情况下的鸡油也有强的抗氧化能力.%An efficient microwave-assisted extraction (MAE) technique was developed to extract antioxidants from Enokitake.The operating parameters were optimized using orthogonal experiment.Scavenging effect of Flammulina extract on DPPH radical was studied, The optimum extraction conditions were as follows: extraction temperature of 75℃, extraction time of 23 min, and solution-solid ratio of 6 (g/mL) ,respectively.The antioxidant activities of the extracts were analyzed, and the results showed that the extracts presented strong ability of radical-scavenging properties against the DPPH and strong reducing power.It also inhibited the peroxidantion of chicken oil.
展开▼
