从口腔中分离得到一株唾液链球菌BD3900,在体外试验中发现,该菌具有极强疏水作用(95.8％)和静电作用(表面电荷量≥88.6％)有利于黏附于口腔中,能耐受100 μg/mL的溶菌酶,存活能力较强;具有较高的自聚性(15％～20％)及与变形链球菌(Streptococcus.mutans)的共聚作用,在蔗糖存在时,与变形链球菌共聚率高达64.3％;与变形链球菌共培养时,可以抑制变形链球菌生物膜的形成,抑制率为67.1％.说明BD3900是一株具有预防龋齿潜力的口腔益生菌.%Interactions between Streptococcus salivarius and Streptococcus mutans play important roles in the development of dental biofilms in oral cavity.S.salivarius BD3900,isolated from the oral cavity of a healthy voluteer,displayed the potential to be an oral probiotics in vitro.Strong hydrophobicity (95.8％) and high surface charges(≥88.6 ％) endure the strain to be easily adhered in oral cavity.The strain BD3900 was tolerant to 100 μg/mL lysozyme(2.0× 104 U/mg),indicating the high ability for the strain to survive in oral cavity.Besides,the strain was determined to be with high autoaggregating ability (15 ％ ～ 20％) as well as co-aggregating abiltity with Streptococcus mutans.in the presence of sucrose at a concentration dependant manner,with a highest co aggregation rate of 64.3％ at a sucrose level of 5 g/100 mL,while no obvious effect of sucrose was observed on the auto-aggregation of the strain BD3526.Furthermore,when co-cultured with the strain BD3900,the formation of S.mutans biofilm could be reduced by 67.1％.Therefore,the strain S.salivarius BD3900 is a candidate of oral probiotics with potential in prevention of dental caries.
展开▼
