利用单因素试验结合响应曲面法(RSM)优化马齿苋多糖的醇沉工艺.在单因素基础上选取试验因素水平,根据中心组合设计原理采用三因素三水平的响应面分析法进行最佳工艺的优化,研究醇沉比、离心时间、离心转速比对马齿苋多糖提取率的影响.在分析各个因素的显著性和交互作用后,得到马齿苋多糖最佳醇沉工艺为在醇沉比为1:4(mL/mL),离心时间为10 min,离心转速为3800 r/min时,提取率为9.5%.%Response surface methodology(RSM)was used to optimization of ethanol precipitation of polysaccha-rides from Portulace oleracea L..Based on the single factor analysis, the effect of centrifugal time, the centrifu-gal rotational speed and solid-to-alcohol ratio on polysaccharides from Portulace oleracea L. was evaluated by response surface analysis. Three factors, three levels central composite experimental design was applied and the interactions of each factor on the polysaccharides from Portulace oleracea L. were also investigated. The centrifugal rotational speed and solid-to-alcohol ratio had significant effects on extraction yield. The optimal conditions of polysaccharides in Portulace oleracea L. alcohol sinking process were as follows:the liquid-to-al-cohol ratio was 1:4(mL/mL),centrifugal time was 10 min, and the centrifugal rotational speed was 3800 r/min. Under such conditions, extraction of the polysaccharides yield was 9.5%.
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