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腰果蛋白的提取及功能特性研究

         

摘要

It focused on the method which called alkali extraction and acid precipitation , the optimized extrac-tion parameters and its functional properties from the protein of cashew nut. Through the orthogonal experiment, the order of the extraction factors was as follows: solid-liquid ratio>time>pH>temperature, the optimized cashew nut protein extraction parameters was:extraction at 55℃for 2 h with solid-liquid ratio 1∶15(g/mL), extraction buffer pH 8.5.Through the study of cashew nut protein functional properties, cashew nut protein iso-electric point pI=4.6,Temperature at 55℃the solubility of protein dissolvability was the maximum. In the con-centration of NaCl solution 0-0.9mol/L, cashew nut protein appeared phenomenon of salt soluble, when NaCl solution concentration reached 1.5mol/L was developing salting-out. At 30℃cashew nut protein water-retain-ing capacity was 3.34 g/g, with the increase of temperature, cashew nut protein water-retaining capacity gradu-ally decreases. Cashew nut protein water-retaining capacity firstly increased and then reduced with the concen-tration of NaCl solution increase, concentration of NaCl at 0.9 mol/L cashew nut protein water-retaining capac-ity was 3.66 g/g. At 30℃protein oil-holding capacity was 1.30 g/g, as the temperature rises cashew nut protein oil-holding capacity gradually reduced.%以腰果为材料,以碱提酸沉法提取腰果中的蛋白,确定最优的提取参数,并探讨了腰果蛋白的功能性质。通过正交试验结果可知,提取腰果蛋白影响因素重要性依次为:料液比>提取时间>提取液pH>温度;最佳提取工艺条件:提取时间2 h,提取液pH8.5,料液比1∶15(g/mL),温度55℃,腰果蛋白提取率为85.25%。对腰果蛋白功能性质的研究表明,腰果蛋白的等电点pI为4.6;温度在55℃时,蛋白质的溶解度达到最大;在浓度为0~0.9 mol/L的NaCl溶液中,腰果蛋白出现盐溶现象,NaCl溶液浓度达到1.5 mol/L时,则产生盐析现象;30℃时,腰果蛋白持水力为3.34 g/g,随着温度的升高,腰果蛋白持水力逐渐下降;随着NaCl浓度的增加,腰果蛋白持水力先增大后减小,NaCl浓度为0.9 mol/L腰果蛋白持水力最大达到3.66 g/g;腰果蛋白在温度30℃时持油力为1.30 g/g,随着温度的升高腰果蛋白的持油性逐渐降低。

著录项

  • 来源
    《食品研究与开发》 |2016年第6期|43-49|共7页
  • 作者单位

    广西高校食品安全与检测重点实验室桂林理工大学化学与生物工程学院;

    广西桂林541004;

    深圳爱康生物科技有限公司;

    广东深圳518131;

    广西高校食品安全与检测重点实验室桂林理工大学化学与生物工程学院;

    广西桂林541004;

    广西高校食品安全与检测重点实验室桂林理工大学化学与生物工程学院;

    广西桂林541004;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类
  • 关键词

    腰果; 提取; 优化; 腰果蛋白; 功能性质;

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