采用纤维素酶辅助从青稞粉中提取淀粉，以淀粉提取率为评价指标，在单因素试验的基础上选择加酶量、酶解时间、酶解温度、pH 4个主要影响因素进行正交试验，确定最佳的提取工艺条件，并将其应用于超声中试放大试验。正交试验结果表明，加酶量、酶解温度以及酶解时间与酶解温度的交互作用对淀粉提取率有显著影响。试验范围内获得的最佳提取工艺条件为：加酶量100 U/g、酶解温度45℃、酶解时间6 h、pH 4.8，此时，淀粉提取率为80.02%。中试放大试验结果表明，正交试验所确定的最佳提取工艺稳定。%Starch was extracted from highland barley flours assisted by cellulose. In order to find the best condi-tions of enzymatic hydrolysis, with the starch extraction yield as the evaluation index, an orthogonal test was adopted to investigate added-amount of cellulase, enzymatic time, enzymatic temperature and pH value based on the results of single-factor tests. According to the best extraction conditions obtained, ultrasonic pilot enlarge experiment was carried out. The result of the orthogonal optimization test showed that added-amount of cellu-lase, enzymatic temperature, as well as the interaction between enzymatic time and enzymatic temperature have significant effects on the starch extraction yield. The best extraction conditions would be:added-amount of cel-lulase was 100 U/g, enzymatic temperature was 45℃, enzymatic time was 6 h and pH value was 4.8, under which the starch extraction yield from highland barley was 80.02%. The result of the ultrasonic pilot enlarge ex-periment indicates that the best extraction conditions obtained by the orthogonal optimization test were stable.
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