采用化学修饰法将高粱蛋白质（SP）制备成硒化高粱蛋白质（Se-SP）并鉴定其抗氧化活性。结果表明，各种Se-SP均表现出一定的谷胱甘肽过氧化物酶（GPX）活力，其中Se-SP23的活力最高，为227 U/g蛋白。各种Se-SP均还可发挥清除1，1-二苯基-2-三硝基苯肼自由基（DPPH·）、羟自由基（·OH）和超氧阴离子（O2-·）等自由基的作用，并可有效降低抗坏血酸/二价铁离子（VC/Fe2+）诱导损伤线粒体中脂质过氧化产物丙二醛（MDA）的产生，维持心肌线粒体的形态，抑制损伤线粒体的膨胀。%Selenide sorghum proteins (Se-SPs) were prepared by chemically modified method, and their an-tioxidant activities were identified. The results showed that Se-SPs possessed certain antioxidant activity of glu-tathione peroxidase (GPX), Wherein, GPX activity of Se-SP23 was highest, about 227 U/g protein. Se-SPs could play a scavenging role in free radical such as 1,1-diphenyl-2-three nitrophenylhydrazine(DPPH·), hy-droxyl radical(·OH) and superoxide anion(O2-·). Moreover, Se-SPs could effectively decrease content of the lipid oxidation product MDA from damaged mitochondria induced by ascorbic acid/divalent iron ions(VC/Fe2+), and could be maintained in the cardiac mitochondrial morphology to avoid swelling.
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