In this paper, GC-MS was used to detect the fatty acids in the milk. The Milk fat was estracted by OP emulsifier, CH3ONa-CH3OH solution as derivatization reagent, isolated by HP-5MS silica capillary column, identified and determined by gas chromatograph equipped with mass spectrometer. The major contituents of the fatty acid which were analyzed by area nomalization were lauric acid 2.11%, myristic acid 9.81%, palmitic acid 28.06%, linoleic acid 3.37%, oleic acid 27.23%, slearic acid 18.82%. The method is simple,accurate and reliable, it is suitable for the detection of fatty acid in the milk. The milk fatty acid have mainly composed of saturated fatty acids, the ration of fatty acid was 62.43%.%采用气相色谱-质谱（GC-MS）测定方法，对生鲜牛乳中脂肪酸的组成和含量进行检测。气相色谱-质谱（GC-MS）测定方法中，生鲜牛乳经OP乳化剂提取脂肪，CH3ONa-CH3OH酯化后用正己烷萃取，通过HP-5MS毛细管柱对其进行分离，测定了生鲜牛乳中脂肪的成分。确定了主要的脂肪酸组成及含量为月桂酸2.11%、豆蔻酸9.81%、棕榈酸28.06%、亚油酸3.37%、油酸27.23%、硬脂酸18.82%等。本方法简单易行，准确可靠，适合牛乳脂肪酸检测；生鲜牛乳脂肪酸主要由饱和脂肪酸组成，含量为62.43%。
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