优选马鞭草中总三萜酸的提取工艺.采用紫外分光光度法测定总三萜酸含量;以总三萜酸得率为指标,采用单因素试验考察乙醇浓度、料液比、提取时间、提取次数的影响,在此基础上设计正交试验优选马鞭草中总三萜酸的提取工艺.最佳提取工艺为80%乙醇35倍量超声提取3次,每次30 min.在此条件下,总三萜酸得率21.91 mg/g.该工艺稳定可行,为马鞭草中三萜酸的开发利用提供参考.%To optimize the extraction technology of total triterpenoids from Verbena officinalis L..The content of total triterpenoids was determined by UV with oleanic as reference; Effects of concentration of extraction solvent,solid-liquid ratio,extraction time and extraction times by taking yield of total triterpenoids as the index,basis on this,extraction technology was optimized by orthogonal test.The optimum extraction technology were as follow:extracted 3 times with 35 times the amount of 80%ethanol,30 min each time.Under these conditions,yield of total triterpenoids from Verbena officinalis L.was 21.91 mg/g.This extraction process was stable and feasible,which provide the reference for the development and utilization of triterpenoids from Verbena officinalis.
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