以0812澡为原料,通过正交试验分别探讨了提取β-胡萝卜素与SOD的最佳工艺。试验结果表明:提取0812藻中β-胡萝卜素的最佳参数为:料液比1∶8 g/mL,提取温度45℃,提取时间10 min,乙醇浓度90%,此时β-胡萝卜素的浓度可达(2.60±0.30)μg/g;提取SOD的最佳参数为:pH 7.4,超声波功率230 W,热处理温度55℃,热处理时间22 min,酶活力为(72.33±0.22) U/g。%The extraction process conditions of β-carotene and SOD in 0812 algae were investigated by extraction method. The results showed that optimum conditions for extractingβ-carotene was as follows, 0812 algae was mixed with ethanol at ratio of 1∶ 8 g/mL, extract temperature is 45 ℃, extract time is 10 min and ethanol consistence is 90%, consistency reach to (2.60±0.30)μg/g;Optimum conditions for extracting SOD was as follows, pH is 7.4, ultrasonic power is 230 W, process temperature and time are 55℃and 22 min, enzymatic activity is (72.33±0.22) U/g.
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