To establish a method for determining oxytetracyline , tetracycline and chlortetracycline residues in meat by HPLC. The sample was extracted with methanol, n-hexane saturated with methanol to remove impurities,and using C18 column (4.6 mm × 150 mm, 5μm), mobile phase was 0.01 mol/L sodium dihydrogen phosphate (containing 0.5%phosphoric acid) solution-acetonitrile=81∶19 (volume ratio),wavelength of detector was 350 nm. Oxytetracycline, tetracycline and chlortetracycline separation were well separated, oxytetracycline, tetracycline and chlortetracycline extraction recoveries were 80.7 %,78.6 % and 80.4 %, respectively. The limit was 0.02 mg/kg for oxytetracyline , 0.02 mg/kg for tetracyline and 0.05 mg/kg for chlortetracycline. This method is simple, accurate and convenient. It is very suitable for the quanlity control of oxytetracyline, tetracyline and chlortetracycline residues in meat.%建立高效液相色谱法测定牛羊肉中土霉素、四环素和金霉素残留量的检测方法。采用甲醇提取，用甲醇饱和的正己烷去除脂溶性杂质，用5%高氯酸沉淀蛋白。采用C18柱（150 mm×4.6 mm，5μm），流动相为0.01 mol/L磷酸二氢钠（含0.5%磷酸）溶液-乙腈=81∶19（体积比），检测波长为350nm。霉素、四环素和金霉素的分离度好，土霉素、四环素和金霉素的提取回收率分别为80.7%、78.6%和80.4%（n=9）。本方法操作简单，结果准确，可以有效地控制牛羊肉中土霉素、四环素和金霉素残留量。
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