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首页> 外文期刊>Food Chemistry >Multiresidue determination of tetracyclines, sulphonamides and chloramphenicol in bovine milk using HPLC-DAD
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Multiresidue determination of tetracyclines, sulphonamides and chloramphenicol in bovine milk using HPLC-DAD

机译：HPLC-DAD法测定牛乳中四环素，磺酰胺和氯霉素的多残留量

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摘要

A high-performance liquid chromatographic method for the simultaneous determination of tetracycline, oxytetracycline, chlortetracycline, sulfamethazine, sulfaquinoxaline, sulfamethoxazole and chloramphenicol in milk has been developed. The determination of these antimicrobials was carried out using HPLC-DAD with a C_(18) hybrid column and gradient elution with a mobile phase composed of an aqueous phase of 0.075 mol L~(-1) sodium acetate, 0.035 mol L~(-1) calcium chloride and 0.025 mol L~(-1) sodium EDTA, pH 7 and an organic phase of methanol:acetonitrile, 75:25 v/v. Sample preparation involved protein precipitation followed by solid-phase extraction using a polymeric cartridge. The method was validated and applied for the analysis of pasteurised milk samples commercialised in Brazil. The limits of quantitation for all antimicrobials, with the exception of choramphenicol, were below the maximum residue limit, which indicates that the method is appropriate for the determination of these antimicrobials in milk.
机译：建立了同时测定牛奶中四环素，土霉素，金霉素，磺胺二甲嘧啶，磺胺喹喔啉，磺胺甲恶唑和氯霉素的高效液相色谱方法。使用带有C_（18）混合柱的HPLC-DAD并使用由0.075 mol L〜（-1）乙酸钠，0.035 mol L〜（- 1）氯化钙和0.025 mol L〜（-1）EDTA钠，pH 7，有机相为甲醇：乙腈，体积比为75:25。样品制备涉及蛋白质沉淀，然后使用聚合物盒进行固相萃取。该方法经过验证，可用于分析巴西商业化的巴氏灭菌牛奶样品。除氯霉素外，所有抗菌药物的定量限均低于最大残留限度，这表明该方法适用于牛奶中这些抗菌素的测定。

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  • 来源
    《Food Chemistry》 |2009年第3期|545-552|共8页
  • 作者

    Monica Cecilia Vargas Mamani; Felix Guillermo Reyes Reyes; Susanne Rath;

  • 作者单位

    Department of Analytical Chemistry, Institute of Chemistry, University of Campinas-UNICAMP, P.O. Box 6154, 13084-971 Campinas, SP, Brazil;

    Department of Food Science, School of Food Engineering, University of Campinas-UNICAMP, P.O. Box 6121, 13083-970 Campinas, SP, Brazil;

    Department of Analytical Chemistry, Institute of Chemistry, University of Campinas-UNICAMP, P.O. Box 6154, 13084-971 Campinas, SP, Brazil;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    antimicrobials; tetracyclines; sulphonamides; chloramphenicol; HPLC; milk;

    机译：抗菌剂四环素;磺酰胺;氯霉素HPLC;牛奶;

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