Aim To establish an UPLC method for the determination of Dehydroacetic acid in food Methods The UPLC system consisted of CAPCELL PAK MG-C18 column, 10 %CH3OH,0.02 mol/L Ammonium acetate (pH7.0-8.0)as a mobile phase, with a detection wavelength at 293 nm. The flow rate was 0.5 mL/min and column temperature was 30 ℃. Results The linear range was between 0 and 200 μg/mL, R2=0.999 4. The recoveries for Dehydroaetic acid products were 97.6%-101.2%. Minimum delectability was 1μg/mL and the RSD was less than 10%. Conclusion The method is simple, accurate, reliable and can be used in the detection of Dehydroacetic acid in food.%建立超高效液相色谱法（UPLC）测定各类食品中脱氢乙酸的方法采用CAPCELL PAK MG-C18柱，10%甲醇-90%0.02 mol/L乙酸铵（pH=7.0～8.5）作为流动相，流速为0.5 mL/min，柱温为30℃，检测波长为293 nm。在0～200μg/mL范围内各组分峰面积与相应浓度呈良好的线性关系，R2=0.9994，加标回收率为97.6%~101.2%，最低检出限为1μg/mL，方法精密度相对标准偏差小于10%。该方法快速、简便、准确、重现性好，分离效能好，适合对食品中脱氢乙酸的测定。
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