The total gypenoside contents of Gynostemma pentagynum Z.P.Wang in three habitats such as Wulingyuan,Jishou and Baojing were determined.The results showed that the differences between the highest and lowest contents of total gypenosides in the stems and leaves of the plant from the different habitats were 17.3 folds and 14.2 folds,respectively.For each habitat,the contents of total gypenosides in the stems and leaves revealed an increasing trend with prolonged seasonal growth period.In addition,the contents of total gypenosides in the leaves of G.pentagynum Z.P.Wang were higher than those in the stems during different growth periods.%测定与分析武陵源区、吉首市和保靖县共3个生境的五柱绞股蓝总皂苷含量。结果提示：不同生境的五柱绞股蓝其茎和叶的最高与最低总皂苷含量分别相差17.3倍和14.2倍,总皂苷含量有随季节生长期延长而递增的趋势。不同生长时期的五柱绞股蓝,其叶的总皂苷含量均高于同期茎的。
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