设计并合成4-苯甲酰基吡唑啉酮（PMBP）缩α-丙氨酸席夫碱配体及过渡金属Ni（II）配合物。采用元素分析、红外光谱、差热-热重、紫外光谱法研究其配位结构和理化性质,测定配体及配合物对大肠杆菌、金黄色葡萄球菌的抑菌性能,并选择配合物对昆明种小鼠进行毒理和致畸实验。结果表明：配体以酮式和烯醇式结构共存,配位时以烯醇式发生羟基去质子化结构同金属离子配合;配体及配合物都表现出较好的抑菌能力,但配合物的抑菌性能明显强于配体,且对两种不同菌体存在显著差异;急性毒理、微核和精子畸形实验均为阴性反应,表明目标配合物对小鼠无毒副和致畸作用。因此,配合物作为新型抑菌剂是安全可行的。%A new ligand containing 1-phenyl-3-methyl-4-benzoyl-5-pyrazolone and α-alanine and its Ni（II） complex was synthesized by refluxing reaction.Their coordination structure and physicochemical properties were characterized using elemental analyzer,IR spectroscopy and UV spectroscopy,their antibacterial activity against Eschetichia coli and Staphylococcus aureus was studied,and their toxicology and teratogenicity of the complex was tested towards KM white mice.The results indicated that the ligand in a keto-form structure could be turned into an enol-form structure after coordination.Both the ligand and its complex had certain antibacterial activity.However,the bacterial activity of the complex was obviously stronger than that of the ligand.Acute toxicity test showed that the complex was non-toxic.Moreover,micronucleus and teratogenicity tests were negative,suggesting that the complex is safe for mammals.Therefore,it is safe and feasible as a new antibacterial agent.
展开▼
