采用同源克隆和末端快速扩增(RACE)方法克隆了建鲤(Cyprinus carpio var.jian)MHC class Ⅰ基因全长cD-NA并进行了序列分析.结果显示:实验得到了1914 bp的建鲤的MHC class Ⅰ全长cDNA序列;建鲤MHC class Ⅰ基因包括1044 bp的开放阅读框(ORF),118 bp的5'非编码区(UTR)以及752 bp的3'非编码区(UTR),含有保守的半胱氨酸残基,N-糖基化位点.氨基酸序列比对结果显示,建鲤MHC class Ⅰ与日本鲤的MHC class Ⅰ相似性最高,为66.0%;与虹鳟、大西洋鲑、青鳉、红鳍东方鲀的相似性分别为54.5%、57.9%、44.3%、42.0%,与小鼠、大鼠、人的相似性分别为29.1%、28.7%、29.7%.%Major histocompatibility complex(MHC) is one of crucial immune factors in vertebrates. The full-length eDNA of the Cyprinus carpio var. jian MHC class I that was cloned by homology cloning and RACE PCR approach is 1914 bp,The sequencing results showed that Cyprinus carpio var. jian MHC class I gene including 1044 bp ORF, 118 bp 5' terminal UTR and 752 bp 3' terminal UTR, and the conserved cysteine residues, N-glycosylation sites. The alignment result of aminoacids sequences showed that the identity between Cyprinus carpio var. jian MHC class I and Japan carp was 66. 0％which was the highest, and the identity with Oncorhynchus mykiss, Salmo salar, Oryzias latipes, Takifugu rubripes was 54.5％, 57.9％, 44.3％, 42.0％, and the identity with Mus musculus, Rattus norvegicus, Homo sapiens was only 29. 1％, 28.7％, 29. 7％, respectively.
展开▼
