利用秀丽线虫模型及DPPH·清除试验对铅绿褶菇进行体内和体外抗氧化活性研究.结果显示,铅绿褶菇发酵液可明显提升秀丽线虫的抗氧化能力, 0. 5 mg·mL -1时,可显著提高秀丽线虫的平均寿命,比空白对照组提高了70%,野生未开伞子实体的抗氧化能力较开伞子实体强;未开伞子实体水提液0.5～1.0 mg·mL -1处理的秀丽线虫平均寿命分别为4.85 d和5.06 d ,显著高于空白对照组.发酵液对DPPH·的清除率最高,达98. 14%,其次是开伞子实体水提液92.86%,未开伞子实体水提液为85. 71%.表明铅绿褶菇可成为抗氧化物开发的潜在生物材料.%Lifespan of Caenorhabditis elegans in vivo ,along with the DPPH radical scavenging test in vitro ,was used to evaluate the anti-oxidative activity of Chlorophyllum molybdites .The results showed that the C .molybdites fermentation broth increased the resistance of the nematodes to oxidative stress .At 0.5 mg · mL -1,the broth extended the mean lifespan of the paraquat-treated C .elegans 70% longer as compared to that of CK .The anti-oxidative activity of the water extract of immature fruitbodies was greater than that of the mature fruitbodies of C . molybdites .When treated with the water extract of immature mushrooms at 0.5 and 1.0 mg · mL -1,the mean lifespans of the nematodes were 4.85 d and 5.06 d ,respectively ,which were significantly longer than that of CK . The DPPH scavenging rate of the fermentation broth was the highest ,at 98.14%,followed by that of the water extract of mature fruitbodies ,at 92.86%,and that of the water extract of immature fruitbodies ,at 85.71% .The findings suggested that C . molybdites could potentially be a new source for producing antioxidant from a biomaterial .
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