采用双层平板法和平板对峙法分别筛选对试验细菌指示菌和植物病原菌具有抑菌效果的内生拮抗真菌,并通过形态特征观察和18SrDNA 序列测定对拮抗真菌进行鉴定。结果表明：从单丛茶树中筛选出 F15GEN、F15YE 和LB35JING 这3株内生拮抗真菌,其中 F15GEN 对金黄色葡萄球菌抑菌圈直径为13.36 mm,F15YE 对枯草芽孢杆菌的抑菌圈直径为14.92 mm,LB35JING 和 F15GEN 对立枯丝核病原菌和梨腐烂病原菌的生长有抑制作用,F15YE 对核桃叶枯病原菌的生长有抑制作用,LB35JING 对茄腐镰刀病原菌的生长有抑制作用。经形态学和18S rDNA 序列鉴定：F15GEN 为斜卧青霉 Penicillium decumbens ,F15YE 和 LB35JING 均属于青霉属 Penicillium sp.。%Endophytic fungi antagonistic against the selected bacteria and phytopathogens were first screened from the microbial samples collected from the single-clump tea plants using the double-plate and the flat-stand methods. Then,they were identified by their morphologies and 18SrDNA sequences.Three strains of the fungi,i. e., F1 5GEN,F1 5Ye, and LB35JING, were isolated. The diameter of the inhibition zone of F1 5GEN against Staphylococcus aureus was 13.36 mm,and that of F1 5YE against Bacillus subtilis 14.92 mm.In addition, LB35JING and F1 5GEN showed inhibition activities against Rhizoctonia solani and Valsa ambiens Pers ,F1 5YE against Sphaerulina j uglandis ,and LB35JING against Fusarium.The 18S rDNA sequence of F1 5GEN identified it as Penicillium decumbens ,while those of F1 5YE and LB35JING linked them to Penicillium sp .
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