目的研究细柱五加果实乙醇提取物对四氯化碳致小鼠急性肝损伤的保护作用。方法将60只小鼠随机分成细柱五加果实的高、中、低剂量组以及阳性对照组、模型对照组、空白对照组共6组。6组小鼠ig给药20 mL/（kg.d）。连续14 d。第14 d给药1 h后,除空白对照组外,其余各组均ip 0.1%CCl4 0.2 mL/20g。所有小鼠在ip CCl4 16 h后采用眼球取血并脱颈椎处死。然后离心取血清,剖腹取肝。分别将各组肝脏取出0.1 g,加0.9 mL生理盐水进行匀浆。检测血清中丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转氨酶（AST）活性及肝匀浆中丙二醛（MDA）含量、超氧化物歧化酶（SOD）活性的变化。结果与模型对照组相比,不同剂量的细柱五加果实乙醇提取物均能显著降低血清中丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转氨酶（AST）的活性及肝组织中丙二醛（MDA）的含量,提高超氧化物歧化酶（SOD）的活性。结论细柱五加果实乙醇提取物对四氯化碳致小鼠的急性肝损伤有保护作用。%Objective： To study the protective effects of the ethanol extracts of the fruits of Acanthopanax gracilistylus on acute liver injury induced by carbon retrachloride in mice.Methods： Sixty mice were randomly divided into the fruits of Acanthopanax gracilistylus high dose group,middle dose group,low dose group,the positive control group,the model group and the normal group.Six groups of mice were administrated orally 20 mL/（kg·d） for 14 days.Except the normal group,other groups were injected 0.1% carbon retrachloride 0.2 mL/20g after 1 hour when the mice were administrated on the 14th day.After all the mice were filled with stomach Carbon tetrachloride 16 hours,blood were obtained by eyeballs and the mice were cervical killed off.Then the serum were separated by centrifuge and the livers were taken by cutting the belly.The livers of each group were cutted 0.1 g and 0.9 mL physiological saline were added to carry on the refining.The change of the Alanine Aminotransferase（ALT）,Aspartate Aminotransferase（AST） in serum and the Malondialdehyde（MDA）,Superoxide Dismutase（SOD） in liver homogenate were examined.Results： Compared with the model group,different doses of the fruits of Acanthopanax gracilistylus could significantly reduce the activity of ALT,AST in serum,the content of MDA in liver homogenate and enhance the activity of SOD in liver homogenate.Conclusion： The ethanol extracts of the fruits of Acanthopanax gracilistylus has obvious effects on acute liver injury induced by carbon retrachloride in mice.
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