Objective To identify the species of Brazilian herb SENE and study on the content of sennoside A and sennoside B, then to compare the results with Chinese Sennae folium, and provide reference for varieties systematics, clinical use and resource exploitation. Methods The source of varieties of Brazilian herb SENE was identified by a technique combined DNA barcoding, geographical origin and morphological features, and the content of sennoside A and sennoside B was analyzed by HPLC. Results DNA barcoding identification indicated that SENE was Senna alexandrina, and its average total content of sennoside A and sennoside B (1. 56% ) was higher than its Chinese counterpart (1. 52% ) and also higher than what is required in Chinese Pharmacopoeia (1. 10% ). Conclusion Brazilian herb SENE derives from Senna alexandrina Mill. , and its sennoside A and sennoside B content have no significant difference with its Chinese counterpart which meets pharmacopoeia requirement.%目的：对巴西草药番泻叶 SENE 进行品种鉴别，进而研究其中番泻苷 A 和番泻苷 B的含量并与中国番泻叶番泻苷 A、B 的比较，为其品种整理、临床使用及资源开发提供参考。方法利用 DNA 条形码—产地—形态分析联用技术鉴定巴西草药番泻叶 SENE 的基原，高效液相色谱技术测定番泻苷 A 和番泻苷 B 的含量。结果品种鉴定结果为番泻叶，其中番泻苷 A 和番泻苷 B 两者的平均总含量为巴西番泻叶(1.56%)>中国番泻叶(1.52%)>2010年版《中华人民共和国药典》(一部)的含量要求(1.10%)。结论巴西草药番泻叶来源于番泻叶 Senna alexandrina Mill.，其番泻苷 A 和番泻苷 B 的含量与中国番泻叶差异不显著，且能达到药典要求。
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