目的：对巴西市场上销售的药用蒲公英的混伪品进行鉴定及生药学研究。方法通过提取样品 DNA、PCR 扩增及双向测序，获得样品的 ITS 序列。采用相似度分析法进行分子鉴定；根据植物产地分布特征和形态特征验证结果，三种方法联用获得鉴定结果；分析混伪品的性状和显微特征。结果 DNA 条形码研究表明巴西药用蒲公英的混伪品来源于菊科植物假蒲公英猫儿菊Hypochaeris radicata 的全草，产地分析和形态分析验证了 DNA 条形码鉴定结果。结论巴西药用蒲公英的混伪品来源于菊科蒲公英属植物假蒲公英猫儿菊 Hypochaeris radicata 的全草。混伪品的生药学特征能够为巴西草药 DENTE DE LEÃO 的真伪鉴别提供依据。%Objective To study a new adulterant of DENTE DE LEÃO in Brazil and perform a pharmaceutical analysi. Methods Total genomic DNA was isolated from the materials,,and nuclear DNA ITS sequences were amplified and sequenced. Similarity identification of BLAST analysis was performed. Next, the results were verified according to plant geographical distribution and morphological profiles. Finally, the features of adulterant were demonstrated through microscopic observation. Results Adulterants of Brazil herbs DENTE DE LEÃO was Hypochaeris radicata Linnaeus, Sp. Pl. The origin analysis and morphological analysis verified the results of DNA barcoding. Conclusion Adulterants of Brazilian herb DENTE DE LEÃO are from the Asteraceae plant Hypochaeris radicata with the whole plants. Adulterants pharmacognosy features can provide the basis for identification of Brazil herbs DENTE DE LEÃO .
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