The inhibitory effect of 14 substances such as kojic acid, ellagic acid and arbutin on tyrosinase was investigated by UV spectrophotometry. The results showed that the inhibition rate of tyrosinase was 96.00 % at the concentration of 1.0 mg/mL with the increase of the concentration. Sunscreen experiment not only through the UV spectrophotometer detection, but also the use of SPF mathematical model calculated 14 kinds of material defense ability, the results of rutin hydrate SPF value of the largest 95.66. Moisture test is to detect the water loss rate over a certain period of time; the experimental results show that the minimum water loss of collagen stock solution is 20.33 %. In general, with whitening, sunscreen, moisturizing multiple effects and relatively good material is rutin hydrate.%美白实验采用紫外分光光度法考察曲酸、鞣花酸、熊果苷等14种物质对酪氨酸酶的抑制作用.实验结果表明,随着浓度的升高抑制率逐渐增大,且在浓度为1.0 mg/mL时,曲酸对酪氨酸酶的最大抑制率最大为96.00 %.防晒实验不仅通过紫外分光光度仪的检测,而且还运用SPF数学模型计算了14种物质的防御能力,结果得到芦丁水合物的SPF值最大为95.66.保湿实验是通过检测在一定时间段内的失水率,实验结果显示胶原蛋白原液的失水率最低为20.33 %.总的来讲,具有美白、防晒、保湿多重功效且相对好的物质是芦丁水合物.
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