An analytical method was established for the determination of the composition of oligoglycerols by using HPLC with ELSD.The optimal conditions were as follows： column was Econosphere Amino（250×4.6 mm,5 μm）,mobile phase： mixture of acetonitrile and water（85：15）,flow rate： 1.0 mL/min,column temperature： 28 ℃,injection volume： 30 μL,drift tube temperature： 32 ℃,flow rate of carrier gas： 1.5 L/min.Under the optimal conditions,the calibration curve was linear over the content of glycerol,diglycerol and triglycerol in the range of 100～300 μg/mL,100～400 μg/mL and 200～600 μg/mL with a correlation coefficient of 0.9979,0.9921 and 0.9969,the limit of quantification（S/N≥10） were 1.5,1.0 and 2.0 μg.The spiked recoveries were in the range of 97 %～103 % with relative standard deviations（RSDs）3.0 %（n=6）.%建立了高效液相色谱-蒸发光散射检测器（HPLC-ELSD）测定低聚甘油组成的方法。以Econosphere Amino（250×4.6 mm,5μm）为色谱柱,乙腈-水（85∶15,V/V）为流动相,流速为1.0 mL/min,柱温为28℃,进样量为30μL,ELSD漂移管温度为32℃,载气流速为1.5 L/min。结果表明,在优化条件下,甘油、二聚甘油和三聚甘油浓度分别在100～300μg/mL、100～400μg/mL和200～600μg/mL范围内呈良好线性,相关系数分别为0.9979、0.9921和0.9969,定量下限分别为1.5、1.0和2.0μg,回收率为97%～103%,RSD〈3.0%（n=6）。
展开▼
