目的：探讨不同浓度尿酸对肾小管上皮细胞氧化应激及凋亡的影响。方法将人近曲小管上皮细胞株(HKC⁃8)随机分为对照组和尿酸A组、尿酸B组和尿酸C组，每组15例，对照组加入培养液，尿酸A组、尿酸B组和尿酸C组分别加入0.1 mmol/L、0.4 mmol/L和0.8 mmol/L尿酸，培养24 h，光泽精化学发光法检测各组肾小管上皮细胞内超氧阴离子生成量，紫外分光光度法检测还原型辅酶ⅡNADPH酶蛋白水平，脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(TUNEL）检测肾小管上皮细胞凋亡率。结果尿酸A组、B组和C组肾小管上皮细胞内超氧阴离子生成量和NADPH酶蛋白水平高于对照组，且呈剂量依赖，差异均有统计学意义(P<0.05)；尿酸A组、B组和C组肾小管上皮细胞凋亡率高于对照组，亦呈剂量依赖，差异均有统计学意义(P<0.05)；尿酸A组、B组和C组肾小管上皮细胞凋亡率分别为(73.4±11.7)%、(322.6±23.2)%、(432.7±34.1)%，均高于对照组的(16.9±1.2)%，差异均有统计学意义(P<0.05)。结论尿酸干预能够影响肾小管上皮细胞内超氧阴离子生成量、NADPH酶蛋白水平和肾小管上皮细胞凋亡率，尿酸浓度越高，超氧阴离子生成量、NADPH酶蛋白水平和肾小管上皮细胞凋亡率越高。%Objective To investigate the effects of different concentrations of uric acid on oxidative stress and apoptosis of renal tubular epithelial cells in vitro. Methods Human proximal convoluted tubule epithelial cell lines (HKC-8) were divided into control group and uric acid group A, uric acid group B and uric acid group C, each of 15 cases, which were cultured with culture medium, 0.1 mmol/L, 0.4 mmol/L and 0.8 mmol/L uric acid for 24 hours. Luci-genin-enhanced chemiluminescence was used to detect superoxide anion levels in renal tubular epithelial cells, and ul-traviolet spectrophotometry was used to measure NADPH levels. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) was applied to detect the apoptosis of renal tubular epithelial cell. Results The su-peroxide anion and NADPH levels in renal tubular epithelial cells in uric acid group A, uric acid group B and uric acid group C were significantly higher than those in the control group, in a dose-dependent manner, P<0.05. The apoptosis rates of renal tubular epithelial cells in uric acid group A, uric acid group B, uric acid group C were (73.4 ± 11.7)%, (322.6±23.2)%, (432.7±34.1)%, significantly higher than (16.9±1.2)%in the control group, P<0.05. Conclusion Uric acid exerts effects on superoxide anion generation, NADPH levels and apoptosis rate in renal tubular epithelial cells, and the higher the uric acid concentration, the higher the superoxide anion generation, NADPH protein levels and the apoptosis rate.
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