Objective To investigate the anti-tumor effect of PTPN12 in Hep-G2 cells, and to reveals the hidden meaning of PTPN12 in the clinical application. Methods The PTPN12 gene was transfected into Hep-G2 cells, and then the anti-tumor effect was analyzed by Real-time RT-PCR, Western Blot, and MTT. Results Increasing PTPN12 expression in IIep-G2 cells were observed. The proliferation of IIep-G2 cells was inhibited, and the apoptosis rate was high. Conclusion PTPN12 may be a novel strategy for bio-therapeutic regents in liver cancer, which can effectively inhibit the growth of tumor cells and promote apoptosis.%目的 探讨抑癌基因PTPN12 在肝癌细胞系Hep-G2 中生物治疗肝癌的效果,揭示PTPN12 在临床应用上的潜在意义.方法 在Hep-G2 细胞中顺转、稳定转染PTPN12 基因后,通过MTT、Western Blot、荧光定量PCR 分析抗肿瘤作用.结果 转染PTPN12 后的Hep-G2 细胞增殖慢,凋亡率高,PTPN12 蛋白表达量高.结论 PTPN12 基因在肝癌表达上调,能有效抑制肿瘤细胞的生长,从而促进肿瘤细胞的凋亡.因此,以PTPN12为靶点开展生物治疗可能是一种新的治疗手段.
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