目的 探讨突变型p53 基因在宫颈癌组织中的表达情况及临床意义.方法 采用聚合酶链(PCR)技术分别对57 份宫颈癌组织、15 份慢性宫颈炎组织的突变型p53 基因表达情况进行检测,并以15 例正常宫颈鳞状上皮组织作对照.结果 突变型p53 基因在宫颈癌、慢性宫颈炎中的表达率分别为71.93%、37.15%.正常对照均为阴性.突变型p53 基因的表达阳性率在宫颈癌病理Ⅲ级中明显高于病理Ⅱ、Ⅰ级( P<0.05) ;在溃疡型中的表达率明显低于菜花型和结节型(P<0.05).结论 采用PCR技术检测宫颈癌中突变型P53 基因的表达,其方法简单,结果直观,有助于预测患者的预后和转归.%Objective To detect the expression of mutant p53 gene in cervical cancer and its clinical significance. Methods Fifty-seven samples of cervical cancer, 15 samples of chronic cervicitis were detected for the expression of mutant p53 gene by polymerase chain reaction (PCR). Fifteen samples of normal cervical squamous epithelial tissues were also detected (the control). Results The expression rates of the mutant p53 gene in cervical cancer, chronic cervicitis were 78.19% and 37.15%. The control showed negative results. The expression rate of p53 protein in cervical cancer of pathological grade Ⅲ was significantly higher than that of pathological grade Ⅱ,Ⅰ(P<0.05). The expression rate in the ulcer type was significantly lower than that in the cauliflower type, nodular type (P<0.05). Conclusion Applying PCR for the detection of mutant p53 gene expression in cervical cancer is simple, intuitive, and useful, which is of great importance to the prognosis of patients.
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