目的：观察丹参酮ⅡA（ Tanshinone ⅡA，TanⅡA）对压力负荷增加大鼠心肌组织核因子-κB（ NF-κB）表达的影响，并探讨其对心肌纤维化的保护作用。方法采用腹主动脉缩窄术制备压力负荷心肌纤维化模型，术后存活32只大鼠随机分成4组（ n ＝8），假手术组、模型组、TanⅡA组（20 mg· kg-1· d-1）及阳性对照药物卡托普利组（100 mg· kg-1· d-1）。术后4周成功造模并开始给药，疗程为4周。8周后检测左心室重量指数、心肌组织病理学、心肌羟脯氨酸含量以及心肌组织NF-κB p65蛋白含量。结果与假手术组比较，模型组大鼠心室治疗指数和左心室质量指数明显升高（ P ＜0．01）；与模型组比较，TanⅡA则显著降低（ P ＜0．01）。与假手术组比较，模型组大鼠心肌羟脯氨酸含量明显升高（ P ＜0．01）；与模型组比较，TanⅡA组则显著降低（ P ＜0．01）。与假手术组比较，模型组大鼠心肌组织NF-κB p65表达显著升高（ P ＜0．01）；与模型组比较，TanⅡA组则显著降低（ P ＜0．01）。结论 TanⅡA能抑制心肌肥厚，减轻心肌纤维化，此作用可能与部分下调NF-κB表达有关。%Objective To observe the effect of tanshinone ⅡA ( Tan Ⅱ A) on expression of myocardial NF-κB in rats with pressure overload , and to explore its protective effects on myocardial fibrosis .Methods The pressure overload myocardial fibrosis models were established by abdominal aorta constriction in Sprague -Dawley ( SD ) rats, these rats were randomly divided into four groups:sham-operation group,model group,Tan Ⅱ A group (20mg· kg-1 · d-1 ) and positive control group (captopril 100mg· kg-1· d-1),with 8 rats in each group.4 weeks after the operation,the animal models were successfully established,then the rats were given drugs,with a course of 4 weeks.After 8 weeks,left ventricular mass index, myocardial tissue pathological changes ,myocardial hydroxyproline content ,myocardial NF-κB p65 expression were detected in rats of the four groups .Results As compared with those in sham -operation group , cardiac mass index and left ventricular mass index were significantly increased in model group ( P <0.01); as compared with those in model group ,these indexes were obviously decreased in Tan Ⅱ A group ( P <0.01).As compared with that in sham -operation group,myocardial hydroxyproline content was significantly increased in model group ( P <0.01),however,as compared with that in model group,myocardial hydroxyproline content was obviously decreased in Tan ⅡA group ( P <0.01).As compared with those in sham-operation group ,NF-κB p65 protein expression levels in myocardial tissues were significantly increased in model group ( P <0.01),however,as compared with those in model group ,which were significantly decreased in Tan Ⅱ A group ( P <0.01).Conclusion Tan Ⅱ A can inhibit cardiac hypertrophy ,relieve myocardial fibrosis,and the effect may be related to down-regulation of the expression of NF-κB.
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