目的:了解深圳市龙岗区腹泻人群中致泻性大肠埃希菌的感染状况及耐药性,为该菌引起腹泻的防控与临床治疗提供依据.方法:收集深圳市龙岗区2011~2015年腹泻人群粪便标本,进行致泻性大肠埃希菌的分离培养、生化鉴定后,利用PCR进行鉴定与分型,然后用K-B琼脂扩散法进行抗生素敏感性测试.结果:致泻性大肠埃希菌的检出率为9.4%(95/1008);年龄分布上存在统计学差异(P<0.05),14~25岁(11.5%)、25~35岁(11.6%)相对较高.检出类型以ETEC为主,占60.0%(56/95),其次为EPEC(22.1%,21/95)和EAEC(15.8%,15/95).出现较高耐药率的是萘啶酸(69.5%),其次是氨苄西林(40.0%)和四环素(34.7%);致泻性大肠埃希菌多重耐药率(MDR)为38.9%,ETEC、EPEC、EAEC多重耐药率分别为25.0%、66.7%、43.8%.结论:深圳市龙岗区五种致泻性大肠埃希菌中 ETEC感染率最高,致泻性大肠埃希菌多重耐药较严重;提示规范合理使用抗生素.%Objective:To survey the infection and drug resistance of diarrheagenic escherichiacoli (DEC) in the population of diarrhea in order to prevention and control of diarrhea caused by bacteria and provide the basis for clinical treatment.Methods:Stool samples of diarrhea patients in Longgang district during the period of 2011-2015 were collected.DEC was isolated,cultured and performed biochemical identification.PCR was used to identify and typing.Kirby Bauer disk diffusion method was applied to perform antibiotic susceptibility testing.Results:The detection rate of DEC was 9.4% (95/1008);There was significant difference (P<0.05) on the age distribution,the top two groups were 14 to 25 (11.5%) and 25 to 35 year-old groups (11.6%).ETEC was the main type accounting for 60.0% (56/95),followed by EPEC (22.1%,21/95) and the EAEC (15.8%,15/95).The rate of resistance to nalidixic acid (69.5%) was highest,followed by ampicillin (40.0%) and tetracycline (34.7%);Multidrug resistance rate of DEC was 38.9%.Those of ETEC,EPEC,EAEC were 25.0%,66.7%,43.8%,respectively.Conclusion:The ETEC infection rate in DEC was the highest in Longgang District.The multidrug resistance of DEC was serious.Therefore clinical doctors should be aware of rational use of antibiotics.
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