确定黑木耳代料栽培培养基软化病的病原菌。根据科赫法则，从黑龙江省和吉林省的多个黑木耳主产区采集病原菌样本，病原菌经分离纯化得到6个菌株，通过对子实体、孢子和菌丝的形态观察及分别进行回接试验确认，从黑木耳代料栽培袋中分离出的致病病原菌其形态与黄孢原毛平革菌Phanerochaete chrysosporium最为相似。为进一步确认该病原菌的分类地位，提取其ITS序列并与该属内15个不同品种进行系统分析。将该菌的ITS序列与GenBank中已知黄孢原毛平革菌Phanerochaete chrysosporium序列AY234893比对，其遗传距离差异为0.0007，相似度为99%，与形态学鉴定结果一致。在东北地区造成黑木耳代料栽培培养基软化病的病原菌为黄孢原毛平革菌。%To identify the pathogen causing flacherie of Auricularia auricular substitute cultivation medium. According to Koch rule,Auricularia auricular pathogens samples were collected in Heilongjiang and Jilin. Pathogens were isolated and purified and six strains were obtained. Through the morphological observation and tieback experiment of fruiting bodies,spores and hyphae,itwas confirmed that the shape of pathogenic bacteria isolated from Auricularia auricular substitute cultivation bag was most similar with Phanerochaete chrysosporium. To further confirm the taxonomic status of pathogenic Bacteria,its ITS sequences was extracted and systematic analysis was conducted with the 15 different species within the genus. The ITS sequences of the bacteria was compared with AY234893 of Phanerochaete chrysosporium known in the GenBank,the genetic distance difference was 0. 0007,the similarity was 99%,consistent with the result of the morphological identification. In the northeast of China,the pathogen causing Auricularia auricular substitute cultivation medium flacherie was Phanerochaete chrysosporium.
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