应用发芽咖啡豆α-半乳糖苷酶法合成α-半乳糖基-β-环糊精,研究其合成工艺.通过BoxBehnken设计等方法,得出工艺条件为α-半乳糖苷酶用量73 nkat,时间28 h,pH 6.3,温度40℃,振荡速度75 r/min,反应体系包括2mL醋酸缓冲液(50 mmoL/L),β-环糊精0.4 mol/L,蜜二糖0.8 mol/L.在此条件下,α-半乳糖基-β-环糊精绝对产率为28.4％,比优化前提高了41.3％.%In view of the problems of the low temperature in north China in winter, low gas digesters, the biogas cell body frost crack and so on, a solution of using solar jointing electric power for the digester heating was proposed. At first, in order to maintain the slurry temperature in suitable range and keep the fluctuation within ±3℃, the digesters and the surrounding soil were considered as an organic whole, a three-dimensional model of temperature control was established. Secondly, the software and hardware of the control system were designed in detail. Finally, the experiment was conducted using an intensive pig farm of 2 000 slaughter pigs per year in Harbin as an example. The results showed that the system needed to joint electric heating in the coldest four months and it could be powered by solar heating alone in other months, the temperature control accuracy of the system was within ±2 ℃.
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