目的：了解大连地区耐甲氧西林金黄色葡萄球菌（M RSA ）的耐药性及其葡萄球菌盒染色体SCCmec的基因型特点。方法采用纸片扩散法检测MRSA对抗菌药物的耐药性；聚合酶链反应（PCR）对SCCmec进行基因分型。结果分离出的38株M RSA菌株均具有多重耐药性，但对万古霉素和氯霉素较为敏感。SCCmec基因型以Ⅱ型最为常见，占86．84％；其次为Ⅲ型，占10．53％；未定型的占2．6％。结论大连地区分离出的M RSA具有多重耐药的特征，SCCmec基因型以Ⅱ型为主。%Objective To investigate antibiotic resistance and Staphylococcal cassette chromosome mec (SCC-mec) genotype of methicillin-resistant Staphylococcus aureus (MRSA)in Dalian Area .Methods Antimicrobial susceptibility of MRSA was detected by Kirby-Bauer method and SCCmec genotype by multiple PCR method .Results 38 strains of MRSA were all multidrug-resistant strains ,but susceptible to vancomycin and chloramphenicol .The most common SCCmec genotype was type Ⅱ (86 .84% ) ,type Ⅲwas in the second place(10 .53% ) ,and unidentified type accounted for 2 .6% .Conclusion MRSA strains in Dalian area are charac-terized by multidrug resistance ,type Ⅱ is the most common SCCmec genotype .
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