Objective To investigate the effect of soluble CD40 ligand (sCD40L) on proliferation of NB4 cells and expressions of interleukin 6 (IL-6) and survivin mRNA.Methods MTT assay was used to examine the effect of different concentrations of sCD40L on leukemia NB4 cells proliferation in vitro at different incubation times;RT-PCR was used to determine the effect of different concentrations of sCD40L on the expression of survivin mRNA in NB4 cells at different incubation times;ELISA was used to detect cytokine IL-6 level in the culture supematant of leukemia NB4 cells of different concentrations of sCD40L at different incubation times.Results MTT assay showed that sCD40L with different concentrations (0.5-2.5μg/ml) could significantly inhibit the proliferation of NB4 cells in a dose-and-time dependent manner (P＜0.05).sCD40L could suppress the expression of survivin and IL-6,and with dose-and-time dependent manner,with statistically significant difference compared with no-treatment control group (P＜0.05).Conclusions sCD40L can significantly inhibit the proliferation of NB4 cells in vitro,yet it is concentration and time dependent.The mechanisms of sCD40L in inhibiting the proliferation of NB4 cells and inducing apoptosis in NB4 cells may be related to their inhibition of IL-6 and survivin expression.%目的 研究可溶性CD40配体(Soluble CD40 Ligand,sCD40L)对人白血病NB4细胞增殖的作用,并探讨其对生存素mRNA(survivin mRNA)及白细胞介素6(IL-6)表达的影响.方法 采用MTT法检测sCD40L孵育NB4细胞体外增殖的影响;通过RT-PCR检测survivin mRNA表达;ELISA测定IL-6的影响.结果 不同浓度(0.5、1.0、1.5、2.0、2.5μg/ml)的sCD40L对NB4细胞随着药物浓度增加、作用时间延长,细胞增殖抑制率上升,差异有统计学意义(P＜ 0.05);sCD40L对NB4细胞survivin及IL-6的表达有明显的抑制作用,与细胞对照组比较差异有统计学意义(P＜0.05),并呈剂量-时间依赖性.结论 sCD40L在体外能够明显抑制NB4细胞增殖,具有剂量-时间依赖性.sCD40L抑制NB4细胞增殖与survivin及IL-6表达相关,并可能与其下调有关.
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