[目的]研究泰山赤灵芝中灵芝酸的检测方法并应用该方法测定灵芝酸A的含量.[方法]利用反相高效液相色谱和电喷雾质谱联用技术,通过研究已知的灵芝酸化合物的相对分子质量及结构特征,鉴定泰山赤灵芝中生物碱提取物中的灵芝酸类化合物,并通过灵芝酸A标准品的标准曲线测定其中灵芝酸A的含量.[结果]在泰山赤灵芝样本中检测到灵芝酸A、A1、B、C1、R、beta、delta、epsilon、gamma、zeta,灵芝酸A的含量为115.4μg/g.[结论]泰山赤灵芝含有较高的灵芝酸,液质联用仪对泰山赤灵芝天然产物结构的分析以及含量测定准确可靠.%[Objective ] The research aimed to study detection method of ganoderma lucidum acid in Taishan Ganoderma lucidum and applica-tion of the method for determining the content of ganoderma lucidum acid A.[ Method] Using reversedp-hase high performance liquid chroma -tography ( HPLC) and electrospray ionization mass spectrometry technology, through the study of ganoderma lucidum acid known compounds and the relative molecular mass of the structure characteristics, identification of ganoderma lucidum acid compounds of Taishan Ganoderma lu -cidum alkaloids in the extracts, and to determining the content of ganoderma lucidum acid A through the standard curve of the standard of gan-oderma lucidum acid A.[Result]We detected ganoderma lucidum acid A, A1, B, C1, R, beta, delta,epsilon , gamma, zeta in Taishan Gan-ode rma lucidu m samples, ganoderma lucidum acid A content of 115.4 μg/g.[Conclusion ] It contents high level ganoderma lucidum acid in Taishan Ganoderma luc idum, liquid chromatograph/mass spectrometer( LC/MS) of TaishanGa noderm a lucidum natural product structure anal-ysis and content determination is accurate and reliable.
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