Objective] To study the preparation and purification of fish oil fatty acid ethyl esters and their effects on blood lipid reduction. [ Method] Fish oil fatty acid ethyl esters were made from fish oil by base catalysis and purified , and the conditions for the ethyl esters produc-tion were optimized.Effects of high levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on blood lipid reduction were studied.[Result] The optimum conditions for ethyl esters production were shown as follows:the molar ratio of ethanol to fish oil was 6∶1;re-action temperature was 75 ℃; reaction time was 2 h;NaOH concentration was 0.30%.After molecular distillation, the yield of the ethyl es-ters was up to 95%under the optimum conditions.DHA and EPA were extracted by silver nitrate-water method, and total content of DHA and EPA was up to 96%-99% when AgNO3 concentration was 75%.High levels of DHA and EPA significantly decreased the contents of serum total cholesterol (TC), triglyceride (TG) and low density lipoprotein cholesterin (LDL-C)and improved the content of high density lipoprotein cholesterin (HDL-C), which indicating that high levels of DHA and EPA could improve the disturbance of blood lipid on hyperlipidemia mice.[Conclusion] The study can provide scientific references for the deep processing, exploitation and utilization of fish oil.%[目的]研究鱼油乙酯的制备、纯化及其降血脂功效。[方法]以鱼油为原料，采用碱催化制备了鱼油乙酯，优化了条件并对其进行纯化，将产品配成饲料进行动物试验，研究其降血脂功效。[结果]试验表明，乙醇－鱼油6∶1（摩尔比），反应温度75℃，反应时间2 h，碱催化剂NaOH加入量为油重的0．30％；在此条件下鱼油脂肪酸乙酯得率最高，经分子蒸馏后达95％；硝酸银最佳浓度为75％，提取得到的二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）总浓度96％～99％；高含量EPA和DHA鱼油能够显著降低小鼠血清中的总胆固醇（TC）、甘油三酯（TG）和低密度脂蛋白胆固醇（LDL-C）浓度，提高高密度脂蛋白胆固醇（HDL-C）浓度，可有效改善高脂血症小鼠的血脂水平。[结论]研究可为鱼油的深加工开发利用提供参考。
展开▼
