[ Objective ] To investigate the expression of leptin and its receptor in the endometrium of buffalo and its effects on embryo implantation. [Method] After female buffaloes by the pregnant mare serum super ovulation mated with bulls, female buffaloes were slaughted at the day ofO, 5, 7, 9 and 15 of pregnancy and the embryo implantation sites of the uterus were removed. By reverse transcription-polymerase chain reaction(RT-PCR) , the expressions of leptin and its receptor ( Ob-Rb) gene in the endometrium of buffalo in the different phases in the process of the embryo implantation were detected. [ Result] Leptin was expressed in the endometrium of buffaloes in the whole process. The expression showed no signficant differences in different phases of the implantation process( P > 0.05). Ob-Rb was also expressed in the endometrium of buffalo in the whole process, but the expressions were significantly different in the different phases of the implantation process (P < 0.05). And the gene expressions of Ob-Rb ascend from day 0 to day 7. the expressions was the highest at 7th day; its expression descent from day 7 to day 15. [Conclusion] Leptin and its receptor may be involved in the embryo implantation and development.%[目的]探讨瘦素及其受体ob-Rb在水牛子宫内膜中的表达规律及其对水牛胚胎附植的影响.[方法]母水牛经孕血清促性腺激素(PMSG)超排后与公牛交配,分别在交配后第O、5、7、9和15天各屠宰10头水牛,分别取胚胎附植部位的子宫组织,提取瘦素及其受体总RNA.利用RT-PCR方法检测不同时期瘦素mRNA及其受体表达情况.[结果]瘦素在胚胎附植过程中在水牛子宫内膜都有表达,各时期的表达水平差异不明显(P＞0.05),Ob-Rb在胚胎附植过程中亦有表达,但是交配后第7、9和15天的表达水平与0和5天差异显著(P＜0.05),并且在妊娠期0～7天内表达水平上升,7天达到峰值,然后下降.[结论]瘦素及其受体Ob-Rb可能不仅参与胚胎的附植,而且也可能参与附植后胚胎的生长发育.
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