[Objective] To establish a microbiological method for assay of vitamin B6 in vitamin drinks.[Method] The Saccharomyces Carlsbrgensis (ATCC 9080) has great sensitivity for vitamin B6 in materials.According to this specificity,it can be assay vitamin B6 in samples.Prepare standard concentrate-response curve by plotting absorbance reading for each level of standard solution.Determine the amount of vitamin B6 activity for each level of assay solution by interpolating from standard curve.[Result] The precision were 1.5％,1.1％ and 1.5％.The RSD was 0.788 7％.The recoveries were all above 96.5％.[Conclusion] Microbiological method can be used to determination of vitamin B12 in vitamin drinks and this method has high sensitivity.The results are precise.%[目的]建立维生素饮料中维生素B6的微生物检测法.[方法]利用卡尔斯伯酵母菌(ATCC 9080)对维生素B6极高的灵敏性和特异性,定量测定出试样中维生素B6含量.即以不同浓度标准溶液的吸光度相对于各浓度水平标准物质的浓度绘制标准曲线,再根据标准曲线计算出试样中维生素B6的含量.[结果]试验方法精密度分别为1.5％、1.1％及1.5％,相对标准偏差为0.788 7％,回收率在96.5％以上.[结论]微生物法可以作为维生素饮料中维生素B6的检测方法,且灵敏度高、测定结果准确.
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