[目的]建立食品中罗丹明B的高效液相色谱-紫外光检测法.[方法]将样品中的罗丹明B用丙酮、正己烷提取,经过氧化铝固相萃取柱净化浓缩后,利用高效液相色谱紫外可见光检测器检测其含量.[结果]罗丹明B在0.005 ～2.000 mg/kg浓度范围内,峰面积与浓度呈良好线性关系,相关系数为0.99998;方法的定量检测限为0.005 mg/kg,且平均回收率高.[结论]该方法适用于食品中罗丹明B的测定,并具有快速、准确、灵敏度高的优点.%[ Objective] The aim was to establish a method for the determination of Rhodamine B in food by HPLC-UV. [ Method] Rhodamine B was extracted with acetone/hexane from food samples. After concentrated and purified by alumina cartridge, the Rhodamine B content in the food was determined by using high performance liquid chromatography with ultraviolet visible detector. [ Result] Within the concentration range of 0.005 -2. 000 mg/kg,the peak area of Rhodamine B presents good linear relation with the concentration with the related coefficient of 0. 999 98. The detection limit of the method is 0.005 mg/kg, and with high average recovery rate. [ Conclusion ] It is a fast and accurate method to detect Rhodamine B in food with high sensitivity.
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