[Objective] To establish n convenient and accurate method for the content determination of polysaccharides from RHIZOMA GAS-rnTRODIAE. [Method] Eatxaction process of RHIZOMA GASTRODIAE polysaccharides was optimized by orthogonal test. The total asccharide content, uronic acid content and protein content were detected by phenol.sulfuric acid method, m-hydroxydiphenyl method, and Comaasie Brilliant method, respectively. [Resuh] The optimal extraction condition was A2B2C3, that is 70 ℃ extraction temperature, 2.5 h extraction time, and 3 extraction times. Under this condition, the extraction rate of polysaccharides reached 13.15%. Saccharide contents in different rngroups of RHIZOMA GASTRODIAE polysaccharides we,re 65.8%, 84.6% and 87.3% ; protein contents were 2.9%, 0. 28% and 2.7% ;rnumnic acid contents were 29.7%, 26. 3% and 39. 6%. [Conclusion] This method was rapid, simple, accurate, and sensitive, and could be used for the quality control of RHIZOMA GASTRODIAE polysaccharidas.%[目的]对天麻多糖的提取工艺进行研究并测定其各组分中多糖、蛋白质和糖醛酸的含量.[方法]用正交试验对天麻多糖的提取工艺进行优选,然后采用苯酚-硫酸法、间羟基联苯法和考马斯亮兰法分别测定天麻多糖及其各组分中总糖、糖醛酸和蛋白质的含量.[结果]最佳提取工艺每件为A2B2C3,即:提取温度为70℃,提取时间为2.5h,提取次数为3次；在此条件下,多糖提取率达13.15％.天麻多糖及其各组分中糖含量分别为65.8％、84.6％和87.3％,蛋白质含量分别为2.9％、0.28％和2.7％,糖醛酸含量分别为29.7％、26.3％和39.6％.[结论]该方法简便、快速、准确,灵敏度高,为天麻多糖生产的质量控制提供了依据.
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