[ Objective ] This study aimed to construct the eukaryotic expression vector of OsVDAC5 from rice and investigate its expression in Pichia pastoris. [ Method ] 0svdac5 gene was ligated to yeast expression vector pPIC3. 5K and inserted into Pichia pastoris GS115 with electro,-poration to obtain positive recombinant yeast strains. 0svdac5 gene was expressed in yeast after induced with 0. 5% methanol, and the specificity of expressed protein was identified by Western Blotting. [ Result] Eukaryotie expression vector 0svdac5: :pPIC3. 5K was successfully constructed and transformed into yeast GS115 for induced expression, and the expressed protein was detected by Western Blotting, which was identified as OsVDAC5. [Conclusion] This study laid the foundation for further investigating the functions of OsVDAC5 protein.%[目的]构建水稻OsVDAC5的真核表达载体,对其进行真核表达研究.[方法]将Osvdac5基因与酵母表达载体pPIC3.5K连接,通过电转化的方法插入到毕氏酵母GS115中,筛选阳性重组菌株,通过0.5％的甲醇诱导使外源的水稻Osvdac5基因表达,并利用Western Blot鉴定表达蛋白的特异性.[结果]成功构建了Osvdac5∶∶pPIC3.5K酵母表达载体,将其整合到酵母GS115基因组后,对其进行诱导表达,产物经Western Blot检测,证实了表达蛋白为OsVDAC5.[结论]该研究结果为进一步研究OsVDAC5蛋白的功能奠定了基础.
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