[Objective] To optimize the extraction technology of poly sac charides from Aloe vera L. var chinensis, compare several purification methods and establish a method for determining the content of polysaccharides. [ Method] With the yield and content of polysaccharides as the evaluation criteria, the extraction process of polysaccharides was optimized by orthogonal experiment. Three polysaccharides purification methods, including dialysis, macroporous resin AB-8 absorption and activated carbon absorption, were compared. The polysaccharides content was detected colorimetrically by the phenol-sulfuric acid method after the conversion coefficient of Aloe vera L. vat ckinensis polysaeeharides to glu-cose was obtained. [Result] The optimal process conditions for extraction were obtained by orthogonal experiment and the optimal parameters were as follows: the solid-liquid ratio of 1:10 g/ml, twice of extraction, and 3 h of extraction time. The contents of polysaccharide purified by the three methods were shown as dialysis > macroporous resin AB-8 > activated carbon; while the yield of polysaccharides was shown as activated carbon > dialysis > macroporous resin AB-8; the conversion coefficient of polysaccharide to glucose was determined to be 1. 32, the average recovery was 98.4% with a relative standard deviation of 2.36%. [Conclusion] The optimized extraction process is economical, simple, stable and practical, the dialysis method is simple, rapid and cheap to be used for purifying the polysaccharides. The phenol-sulfuric acid method is simple and accurate with good reproducibility.%[目的]优化中华芦荟多糖的提取工艺,比较几种精制方法,并建立多糖含量的测定方法.[方法]以多糖得率和含量为评价指标,采用正交试验法对多糖提取工艺进行研究,并比较透析法、AB-8型大孔树脂吸附法和活性炭吸附法3种多糖精制方法；采用芦荟多糖标准品测得芦荟多糖对葡萄糖的换算因子后,用苯酚-硫酸法测定多糖含量.[结果]中华芦荟多糖的最佳提取工艺为料液比1∶10g/ml,提取2次,提取时间3h;不同精制方法得到的多糖含量:透析法＞AB-8型大孔树脂吸附法＞活性炭吸附法,多糖收率:活性炭吸附法＞透析法＞ AB-8型大孔树脂吸附法;苯酚-硫酸法测定芦荟多糖对葡萄糖的换算因子为1.32,平均回收率为98.4％.相对标准偏差为2.36％.[结论]优选工艺经济、简单、稳定、可行,透析法精制芦荟多糖简单快速、成本低、多糖含量高,苯酚-硫酸法简便、准确、重现性好.
展开▼
