[ Objective ] The aim was to explore the transformation activity of hydroxylation enzyme from hydroxylation enzyme producing bacteria Stenorophomonas maltophilia CGMCC 1. 1788. [Method] Biotransformation conditions of hydroxylation enzyme producing bacteria S. Maltophilia CGMCC 1.1788 to pesticide imidaclothiz were optimized by using shaking flask fermentation method. [Result] The converting conditions were established as followed: the best outfit fluid volume was 10 ml per 100 ml conical flask; the best reaction temperature was 25℃; the optimum pH was 6.5; the optimum reaction substrate initial concentration was 0. 2 g/L; and the best reaction time was 48 h. [ Conclusion] The research results provide theoretical basis to play the microbial transformation function of S. Maltophilia CGMCC 1.1788 and understand the biological characteristics of hydroxylation enzyme.%[目的]探究羟基化酶产生菌Stenorophomonas maltophilia CGMCC 1.1788菌株中羟基化酶的转化活性.[方法]采用摇瓶发酵转化方法对羟基化酶产生菌S.maltophilia CGMCC 1.1788对农药氯噻啉的生物转化条件(不同生长时期的细胞、温度、pH等影响因子)进行了优化.[结果]确立了转化条件:100 ml锥形瓶最佳装液量10 ml,最佳反应温度25℃,最适pH 6.5,最佳反应底物起始浓度0.2 g/L,最佳反应时间48 h.[结论]为较好地发挥该菌的微生物转化功能及了解羟基化酶的生物学特性提供了理论依据.
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