[Objective]To distinguish Gentiana straminea Maxim. from wild and cultivated plants by comparing chloroplast DNA psbA-trnH sequences,so as to provide a molecular basis for orgin identification and quality evaluation. [ Method ] cpDNA psbA-trnH sequences of Gentiana straminea Maxim. were amplified by polymerase chain reaction (PCR) ,and then sequenced by direct PCR sequencing method for homologous analysis. [Result] The lengths of cpDNA psbA-trnH of wild and cultivated plants were 316 and 317 bp respectively,and there were 4 variable sites. [Conclusion] The nucleotide differences of psbA-trnH regions could be used for distinguishing Gentiana straminea Maxim. from wild and cultivated plants.%[目的]分析野生与栽培麻花艽(Gentiana straminea Maxim.)psbA-trnH 序列的差异,为麻花艽的基源鉴定和品质评价提供分子依据.[方法]采用PCR 扩增纯化后直接测序的方法,测定野生与栽培麻花艽 cpDNA psbA-trnH 核苷酸序列并作序列同源性分析.[结果]野生与栽培麻花艽的cpDNA psbA-trnH 片段长度分别为316和317 bp,两者之间有4个碱基的变异.[结论]利用psbA-trnH 区序列的差异可以鉴别野生与栽培麻花艽.
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机译：青海栽培黄管秦艽的叶绿体DNA psbA-trnH核苷酸变异和遗传分析Study on Chloroplast psbA-trnH Nucleotide Variation and Genetic Differentiation in Cultivated Plants of Gentiana officinalis from Qinghai
