[目的]研究从发芽咖啡豆中分离纯化α-半乳糖苷酶.[方法]通过硫酸铵沉淀、DEAE-Sepharose Fast Flow阴离子交换色谱和Sephacryl S-200-HR凝胶过滤色谱等分离纯化技术,从发芽咖啡豆中分离纯化α-半乳糖苷酶.SDS-PAGE用来分析该酶的纯度和分子量.[结果]经过3步分离纯化之后,发芽咖啡豆α-半乳糖苷酶的比酶活达到191.50 nkat/mg,纯化倍数为23.18倍.与绿咖啡豆α-半乳糖苷酶相比,发芽咖啡豆α-半乳糖苷酶的比酶活较高,但产率和总的提取率都较低.SDS-PAGE分析结果表明,该酶出现单一谱带,分子量为3 8805 D.[结论]发芽咖啡豆α-半乳糖苷酶产量和总提取率的提高有待进一步的研究.%[Objective] To investigate antioxidant activities of S.pallidfum polysaccharides(SPPS) ire vitro. [Method] H2O2/Fe2+ system,py-rogallol system,LPO system were employed to evaluate SPPS ability to scavenge hydroxyl.LPO and superoxide radical. [Result] 26% hydroxylradical,80% superoxide radical and 40% LPO could be eliminated by 200 μg/ml SPPS. [Conclusion] SPPS were potential anti -oxidantsources.
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